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Recently, a number of light microscopy techniques and microscope modalities have been developed that rely on super-resolution techniques to obtain images with sub-diffraction resolution. These techniques are based on photo-induced switching of the fluorescence emission lifetime of a fluorophore. In the near-field scanning optical microscopy (NSOM) technique, an aperture of the order of 100 nm is required to perform an optical switching event. Therefore, high spatial resolution of this technique is strongly dependent on the aperture size and could potentially be improved by decreasing it. The use of phase-retrieval algorithms for NSOM and X-ray microscopy opens new ways to perform super-resolution experiments, especially for coherent imaging at the X-ray free-electron laser beamlines.The best place to start for followers on Instagram, Facebook and Twitter is their associated app. For Instagram, the app is available for iOS, Android, Windows, and Mac. And it should be noted that Instagram is a free app, unless you want to upload high-res photos.
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Instagram
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